recombinant mpxv antigen a5  (Native Antigen Inc)

Journal: The Lancet. Microbe

Article Title: Assessment of MpoxPlex, a high-throughput and multiplexed immunoassay: a diagnostic accuracy study

doi: 10.1016/j.lanmic.2024.100987

Figure Lengend Snippet: Antigen MFIs of presumed negative samples, post-MPXV infection samples, and post-MVA-BN vaccination samples (A) Sample MFIs run on the multiplexed mpox assay for each antigen. Each graph represents the results for an individual antigen. Each cross represents the MFI for one sample. Significance compared with the presumed negative samples was calculated using an unpaired Dunn’s multiple comparisons test. (B) The ratios of the MFIs for MPXV and VACV antigen homologues MPXV antigen B6 and VACV antigen B5, MPXV antigen A35 and VACV antigen A33, and MPXV antigen A29 and VACV antigen A27 for post-MPXV infection and post-MVA-BN vaccination samples were compared. The significant difference between the two groups was assessed using an unpaired Mann–Whitney test. Each circle represents the ratio of a single sample. There were 435 presumed negative samples, 24 post-MPXV infection samples, and 39 post-Imvanex vaccination samples (14–157 days after the second dose). The sample MFIs for the antigen on the left were divided by the sample MFIs for the antigen on the right (eg, MPXV antigen B6:VACV antigen B5). MFI=median fluorescence intensity. MPXV=monkeypox virus. MVA-BN=modified vaccinia virus Ankara-Bavarian Nordic. VACV=vaccinia virus.

Article Snippet: Recombinant MPXV antigen B2 was purchased from Abbexa (Cambridge, UK; product code ABX620113) and recombinant MPXV antigen A5 was purchased from The Native Antigen Company (Kidlington, UK; product code REC32033).

Techniques: Infection, MANN-WHITNEY, Fluorescence, Virus, Modification

Journal: The Lancet. Microbe

Article Title: Assessment of MpoxPlex, a high-throughput and multiplexed immunoassay: a diagnostic accuracy study

doi: 10.1016/j.lanmic.2024.100987

Figure Lengend Snippet: Individual antigen ROC curves (presumed negative samples vs post-MPXV infection or post-MVA-BN vaccination samples) Each solid line represents the ROC curve for an individual antigen. (A) ROC curves of the MFIs of presumed negative samples (n=435) vs post-MPXV infection samples (n=24). (B) ROC curves of presumed negative samples vs post-MVA-BN vaccination samples (n=39, 14–157 days after the second dose). (C) ROC curves of post-MPXV infection vs post-MVA-BN vaccination samples of each of the 12 MPXV and VACV antigens tested. MFI=median fluorescence intensity. MPXV=monkeypox virus. MVA-BN=modified vaccinia virus Ankara-Bavarian Nordic. ROC=receiver operating characteristic. VACV=vaccinia virus.

Article Snippet: Recombinant MPXV antigen B2 was purchased from Abbexa (Cambridge, UK; product code ABX620113) and recombinant MPXV antigen A5 was purchased from The Native Antigen Company (Kidlington, UK; product code REC32033).

Techniques: Infection, Fluorescence, Virus, Modification

Journal: The Lancet. Microbe

Article Title: Assessment of MpoxPlex, a high-throughput and multiplexed immunoassay: a diagnostic accuracy study

doi: 10.1016/j.lanmic.2024.100987

Figure Lengend Snippet: Summary of ROC curves and MFI cutoffs for presumed negative samples (n=63) vs positive samples (n=435) to 12 MPXV and VACV antigens by ROC analysis

Article Snippet: Recombinant MPXV antigen B2 was purchased from Abbexa (Cambridge, UK; product code ABX620113) and recombinant MPXV antigen A5 was purchased from The Native Antigen Company (Kidlington, UK; product code REC32033).

Techniques:

Journal: The Lancet. Microbe

Article Title: Assessment of MpoxPlex, a high-throughput and multiplexed immunoassay: a diagnostic accuracy study

doi: 10.1016/j.lanmic.2024.100987

Figure Lengend Snippet: Summary of ROC curves and MFI cutoffs for presumed negative samples (n=435) and post-MVA-BN samples (n=39, 14–157 days after the two doses) vs post-MPXV infection samples (n=24) to MPXV antigens A27, A29, and A5 and VACV antigen A27 by ROC analysis

Article Snippet: Recombinant MPXV antigen B2 was purchased from Abbexa (Cambridge, UK; product code ABX620113) and recombinant MPXV antigen A5 was purchased from The Native Antigen Company (Kidlington, UK; product code REC32033).

Techniques: Infection

Journal: The Lancet. Microbe

Article Title: Assessment of MpoxPlex, a high-throughput and multiplexed immunoassay: a diagnostic accuracy study

doi: 10.1016/j.lanmic.2024.100987

Figure Lengend Snippet: Summary characteristics of ROC curves and positivity score cutoffs generated by comparing the positivity scores of all positive samples (n=63) and all negative samples (n=435) to 12 MPXV and VACV antigens by ROC

Article Snippet: Recombinant MPXV antigen B2 was purchased from Abbexa (Cambridge, UK; product code ABX620113) and recombinant MPXV antigen A5 was purchased from The Native Antigen Company (Kidlington, UK; product code REC32033).

Techniques: Generated

Journal: The Lancet. Microbe

Article Title: Assessment of MpoxPlex, a high-throughput and multiplexed immunoassay: a diagnostic accuracy study

doi: 10.1016/j.lanmic.2024.100987

Figure Lengend Snippet: Longitudinal IgG responses to multiple MPXV and VACV antigens following MVA-BN vaccination MFIs of presumed negative samples and post-MVA-BN samples 24 days after dose one and 14–240 days after dose two to 12 MPXV and VACV antigens. Each graph represents the results for an individual antigen. Each point represents geometric mean MFI with 95% CIs for all samples. Significance compared with the presumed negative samples was calculated using an unpaired Dunn’s multiple comparisons test. Samples were taken from individuals who were not vaccinated (n=435); individuals 24 days after one vaccine dose (n=7); and individuals 14 (n=8), 43 (n=10), 63 (n=7), 84 (n=7), 122 (n=7), 157 (n=7), 185 (n=6), 224 (n=8), and 240 (n=8) days after two vaccine doses. MFI=median fluorescence intensity. MPXV=monkeypox virus. MVA-BN=modified vaccinia virus Ankara-Bavarian Nordic. VACV=vaccinia virus.

Article Snippet: Recombinant MPXV antigen B2 was purchased from Abbexa (Cambridge, UK; product code ABX620113) and recombinant MPXV antigen A5 was purchased from The Native Antigen Company (Kidlington, UK; product code REC32033).

Techniques: Fluorescence, Virus, Modification

Journal: The Lancet. Microbe

Article Title: Assessment of MpoxPlex, a high-throughput and multiplexed immunoassay: a diagnostic accuracy study

doi: 10.1016/j.lanmic.2024.100987

Figure Lengend Snippet: IgG responses following two doses of MVA-BN and after MPXV infection MFIs of samples 84 days after two doses of MVA-BN (n=7) and 81 days after MPXV infection (n=22) to 12 MPXV and VACV antigens. Each graph represents the results for an individual antigen. Each point represents the geometric mean of MFIs with 95% CIs for all samples. Significance compared with the presumed negative samples was calculated using an unpaired Mann–Whitney test. MFI=median fluorescence intensity. MPXV=monkeypox virus. MVA-BN=modified vaccinia virus Ankara-Bavarian Nordic. VACV=vaccinia virus.

Article Snippet: Recombinant MPXV antigen B2 was purchased from Abbexa (Cambridge, UK; product code ABX620113) and recombinant MPXV antigen A5 was purchased from The Native Antigen Company (Kidlington, UK; product code REC32033).

Techniques: Infection, MANN-WHITNEY, Fluorescence, Virus, Modification